The youth team will take part in their first away game of the season as they head to Notts County.
Rovers were the only team in the division to win their opening fixture last Saturday as they put Grimsby Town to the sword.
The result shot them to the top of the table at this early stage but their next challenge comes in the form of a team who beat them on the final day of the 2018/19 season.
The Magpies have not started their season yet, so Rovers will be facing an unknown quantity at the weekend.
Kick-off is 11am at Basford United, Greenwich Avenue, NG6 0LD.