Grant McCann insists his side will move on quickly from Friday's defeat at Sunderland as Rovers look to clinch a play-off spot.
Rovers fell to a 2-0 defeat at the Stadium of Light, but know their future is in their own hands as they head into the final three games of the regular season.
McCann said: "We haven't got time to dwell on it and we certainly won't get down about it, we've got another three tough games coming up starting on Tuesday at home to Accrington Stanley and we've got to respond.
"I thought we were a wee bit better in the second half. Ali Crawford and Alfie May added a bit of energy for us, but in the first half the team was tentative and that surprised me a bit."
Sunderland were the better team in the first-half and deserved their two-goal lead at the break, but an injury-time Danny Andrew free-kick which hit a post gave Rovers hope for the second 45 minutes.
McCann was also frustrated that official Andy Woolmer did not give fouls when John Marquis was taken down just outside the area and Andy Butler was pulled down inside the box from a free-kick.
He added: "In the first half we were nowhere near it, Sunderland deserved to be 2-0 up and maybe more - they were far better than us and our boys are disappointed because they haven't done themselves justice and didn't show what we were about.
"Throughout the game we were a bit safe and, by that, I mean we didn't get on the front foot and we didn't get forward or slip people in. Sunderland set up to stop us from getting in behind, but we didn't threaten anywhere near enough for my liking.
"We didn't get the decisions. I thought it was a penalty against Andy Butler but the referee didn't give it and that's the way it goes. Sometimes you need that bit of luck in a place like the Stadium of Light, but we didn't get that."
Tickets for Rovers next Sky Bet League One home game against Accrington Stanley on Tuesday April 23 are still available via the Club Doncaster Box Office. Purchase yours now by either visiting in store at the Keepmoat Stadium, by calling on 01302 762576, or online at tickets.clubdoncaster.co.uk.