Paul Stancliffe believes Friday's defeat to Bradford City will give his U18s side a chance to show their strength of character and bounce back from a set back.
The Bantams were the first team to defeat Rovers this season and Stancliffe praised Bradford City for their performance in their 2-0 on Saturday September 7.
Stancliffe said: "The best team won today - it is as simple as that. They caused us far too many problems in the first-half and they worked really hard from the go which is always going to cause you problems.
“We had spoken all week about how Bradford were one of the best teams we had played last season, certainly when we visited them they put on a good show.
"We knew what to expect coming into this and we knew their strengths but it is disappointing that we made it a bit easier for them today."
The U18s manager thinks the result will enable him to learn more about the characters in his squad by seeing how they react against Mansfield in the Youth Alliance Cup.
He added: "We will watch the game on Monday and go through individual improvements that we need to make but there is no panic from me.
"From the opening five games we have seen what this group of lads are capable of and I have said it before they some of the best players I've worked with in a long time.
"In defeat we will learn about the character of the lads and I'm sure what we will see through trianing next week is a determined bunch who want to set the record straight against Mansfield."