Paul Stancliffe’s U18 side will begin their EFL Youth Alliance Cup campaign against Preston North End U18s on Wednesday.
It will be the first of two group games Rovers will take part in with the second being away at Bolton in November 2020.
Last season the youngsters didn't make it out of the group stage after finishing third place. They fell three points short of Fleetwood Town and Rotherham who qualified through to the next round but came ahead of Mansfield and Carlisle.
The game will take place behind closed doors at Cantley Park.