Rovers U18s start their FA Youth Cup journey with a trip to Basford United on Friday night in the first round proper.
Last season Rovers went on an inspiring run which saw them beat Coventry Sphinx and category two outfit Hull City before falling to defeat against a strong Peterborough United.
Basford also enjoyed an impressive cup run with wins over Port Vale and Rovers’ league rivals Grimsby Town.
U18s boss Gary McSheffrey believes Basford could easily compete in Rovers’ division.
He said: “They are tidy in possession and organised out of it. It’s a good club that would be comfortable in our category three games programme.
“It’s not going to be an easy game. They’re probably better in possession than Rotherham United and Harrogate Town who we have played recently.
“We are going to have to win our individual battles. They are going to be really up for it. They will turn us over if we don’t take them seriously enough.”
Academy manager Tony Cook has stressed the importance of Rovers U18s trying to enjoy the experience.
He said: “Basford are a good side. Last year they beat Port Vale and Grimsby Town before being knocked out of the competition by West Bromwich Albion who ultimately went on to the semi-final of the competition. They are no fools and I am expecting a really tough game.
“Our lads see the FA Youth Cup as the biggest thing in their scholarship programme, which can cramp them up a little bit. It is important that in the build up to it we prepare as professionally as we can but make it enjoyable for them.”
Fans unable to attend the game can follow live through Basford's commentary for free here.
The game will kick off at 7:30pm on Friday November 5 at Greenwich Avenue. Tickets can be purchased here.