Rovers recorded their first defeat of the season in Sky Bet League Two to promotion-chasing Mansfield Town.
Gary McSheffrey named an unchanged side to the one that beat Northampton Town in Sky Bet League Two.
Rovers had a better start than Mansfield but it was a goal on the volley from Stags captain Ollie Clarke that put the visitors in the lead 14 minutes into the match.
George Miller worked his way into a threatening position for Rovers, but in the early phase of the game struggled to get his crosses and shots off.
After going behind Rovers continued to be the better team with Kyle Hurst having a good chance and put a couple of good balls into the box.
The best chance of the first half for Rovers came the way of Miller whose headed effort on goal was just tipped over the bar by Christy Pym.
Mansfield came close to a second but Kyle Knoyle cleared off the line before Jonathan Mitchell attempted to make a save, but was fouled in the process.
Seconds before half-time Harrison Biggins was fouled in the box, and the referee did not hesitate in awarding a penalty. Lee Tomlin was the man to take it this time, and he sent Pym the wrong way.
It deservedly was one a piece at half-time.
Five minutes into the second-half, Kyle Hurst came close for Rovers as he hit the post. A few inches to the right and it would have beaten Pym.
However, it was Mansfield who scored next as they retook the lead through George Maris. There was questions over the goal as there was a foul on Rovers in the build-up.
McSheffrey wasted no time making a double change for Rovers with Jon Taylor and James Maxwell coming on for Harrison Biggins and Luke Molyneux.
Brilliant chance for Rovers to equalise through Kyle Hurst, but Pym could only divert the ball into the path of Tomlin who was fouled. The referee did not give Rovers a penalty and instead showed Tomlin a yellow card.
From one end to another and Mansfield won a penalty. Jonathan Mitchell went the correct way but was still beaten by Lucas Akin's powerful shot from the spot.
Kieran Agard came on for Lee Tomlin on 77 minutes and had two good chances, but was unable to find the back of the net.
Despite any late attempts from Rovers it ended in defeat for McSheffrey's side.
Rovers will look to respond with a trip to Hartlepool United on Saturday, September 10.