Assistant boss Cliff Byrne says there is a real determination at Rovers to progress in the Bristol Street Motors Trophy.
Rovers host Burton Albion of Sky Bet League One in the final Northern Group H game at the Eco-Power Stadium, knowing victory is essential.
“We want to try to progress out of this group, and we need to be at the top of our performance levels and look to get the win to put us on six points in the group,” Byrne said.
“It’s going to be a challenging game against a Burton side on a good run of form. But we believe we have the capabilities and personnel to go out and perform and when you perform, hopefully you get the result.
“It’ll be a really strong, stiff test. They’re going really well in the league at the moment and their form is good.
“We’ve got to focus on us and how we see the opportunities coming our way in the game but make no mistake about it, it’ll be a difficult game.”
A win for Rovers will put them in the driving seat for a place in the knockout stages but may not guarantee progression.
Rovers, Burton and Mansfield Town are all currently on three points in the group, with the Stags and the Brewers still to meet. Mansfield host Everton U21 on Tuesday night.
When Rovers faced Mansfield in the competition last month, there were eye-catching performances from youngsters within the squad such as Jack Goodman, Tavonga Kuleya and Will Flint.
And Byrne says it is necessary that players show they are not merely making up the numbers.
“It’s an expectation from the staff and those players get those minutes because they’ve warranted them,” he said. “They’ve worked hard, they’ve shown signs on the training ground of what they’re capable of.
“You want them to go out and flourish and I felt they did themselves justice on the night, albeit we didn’t come away with the result on the night.”